Selection of W42 recombinant Proteins

Enzyme / Protein	Productivity	Source	Comment
D-amino acid oxidase	180 U/ml, app. 30 g/l	Trig. variabilis
Glutaryl-7-ACA acylase	180 U/ml, app. 30 g/l	Pseudomonas sp.
Penicillin G acylase	29 U/ml, app. 16 g/l	Achromobacter sp.	under development
Penicillin G amidase	64 U/ml, app.   3 g/l	E. coli
Penicillin G amidase	66 U/ml, app.   3 g/l	Prov. rettgeri
Amino acylase I	232 U/ml, app.   5 g/l	Porcine
D-hydantoinase 1	458 UHydantoin/ml 627 UPhenylhydantoin/ml 77 Up-hydroxyphenylhydantoin/ml app. 17 g/l	Bacillus thermoglucosidasius
D-hydantoinase 2	449 UHydantoin/ml 252 UPhenylhydantoin/ml, 9 Up-hydroxyphenylhydantoin/ml app. 12 g/l	Thermus sp.
D-carbamoylase	11 - 12 U/ml, app. 8 g/l	Burkholderia pickettii
Lipase type B two variants	CAL-B1:     14.4 kUTributyin/ml 5.4 g/l CAL-B2:      8.2 kUTributyin/ml 4.9 g/l	Candida antarctica
Serum albumin	6.5 g/l	Human	under development
Protein A (modified) three variants	3 - 6.5 g/l	synthetic
CP-C Esterase	> 400 U/ml, app. 15 g/l	Bac. subtillis	under development
Glutathion (GSH)	6 g/l

intracellular expression = protein in culture volume in g/l