|
biocatalyst |
activity |
assay |
|
D-amino acid oxidase |
78 U/gsupply form, 162 U/gdry
100 – 300 µm |
20 mM Ceph C, 1 bar O2,
100 mM KiPO4, pH 8.0, 25°C |
|
glutaryl-7-ACA acylase |
82 U/gsupply form, 176 U/gdry
100 – 300 µm |
20 g/l Gl-7-ACA, 5 mM KiPO4,
pH 8.0 by 100 mM NaOH, 37°C |
|
Achromobacter sp. Acylase 1 |
217 U/gsupply form, 717 U/gdry
100 – 300 µm |
50 g/l Pen G, 20 mM KiPO4,
pH 8.0 by 100 mM NaOH, 28°C |
|
Achromobacter sp. Acylase 2 |
99 U/gsupply form, 289 U/gdry
200 – 500 µm |
50 g/l Pen G, 20 mM KiPO4,
pH 8.0 by 100 mM NaOH, 28°C |
|
Penicillin G amidase E. coli |
142 U/gsupply form, 333 U/gdry
100 – 300 µm |
50 g/l Pen G, 0.625 mM KiPO4,
pH 8.0 by 100 mM NaOH, 28°C |
|
Penicillin G amidase
Providencia rettgeri |
119 U/gsupply form, 372U/gdry
200 – 500 µm |
50 g/l Pen G, 0.625 mM KiPO4, pH 8.0 by 100 mM NaOH, 28°C |
|
Penicillin G amidase evolved
Providencia rettgeri |
180 U/gsupply form, 581U/gdry
100 – 300 µm |
50 g/l Pen G, 0.625 mM KiPO4, pH 8.0 by 100 mM NaOH, 28°C |
|
Amino acylase I porcine |
101 U/gsupply form, 306 U/gdry
100 – 300 µm |
100 mM NAcMet,
100 mM KiBO3,
pH 7.0, 37°C |
|
Cephalosporin C –
acetyl hydrolase
Bacillus subtilis |
211 U/gsupply form, 622 U/gdry
100 – 300 µm |
75 mM Ceph C, 10 mM KiPO4,
pH 8.0 by 100 mM NaOH, 25°C |
|
Lipase Candida antarctica type B1
Lipase Candida antarctica type B2 |
1261 U/gdry, 100 – 300 µm
1197 U/gdry, 100 – 300 µm |
20 mM tributyrin,
10 mM KiPO4, pH 7.0 by
100 mM NaOH, 25°C |
|
Lipase Candida antarctica type B1
Lipase Candida antarctica type B2 |
2364 U/gdry, 100 – 300 µm
2108 U/gdry, 100 – 300 µm |
20 mmol myristic acid,
20 mmol myristyl alcohol, 25°C |
|
Lipase Candida antarctica type B1
Lipase Candida antarctica type B2 |
17.0 kU/gdry, 100 – 300 µm
57.4 kU/gdry, 100 – 300 µm |
1 mM p-nitrophenylbutyrate,
Triton, IPA, 50 mM KiPO4,
pH 7.0, 25°C |
|
Lipase Candida antarctica type B1
Lipase Candida antarctica type B2 |
635 U/gdry, 100 – 300 µm
468 U/gdry, 100 – 300 µm |
2 mmol phenylethanol,
20 mmol vinylacetate, 25°C |
|
Trypsin bovine |
9.5 U/gsupply form, 23 U/gdry
100 – 300 µm |
2 mM BAPNA, 20 mM CaCl2,
50 mM Tris, pH 7.6, 25°C |
|
D-hydantoinase
Bacillus thermoglucosidasius |
100 – 300 µm
213 U/gsupply form, 539 U/gdry
615 U/gsupply form, 2268 U/gdry |
80 mM hydantoin,
1 mM MnCl2, 100 mM Tris,
pH 8.5, 37°C |
|
D-hydantoinase
Bacillus thermoglucosidasius |
100 – 300 µm
107 U/gsupply form, 344 U/gdry
390 U/gsupply form, 1439 U/gdry |
80 mM phenylhydantoin,
1 mM MnCl2, 100 mM Tris,
pH 8.5, 37°C |
|
D-hydantoinase
Bacillus thermoglucosidasius |
100 – 300 µm
13.0 U/gsupply form, 42.0 U/gdry
9.5 U/gsupply form, 35.0 U/gdry |
80 mM p-hydroxyphenylhydantoin
1 mM MnCl2, 100 mM Tris,
pH 8.5, 37°C |
|
D-carbamoylase
Burkholderia pickettii |
100 – 300 µm
13.9 U/gsupply form, 49.1 U/gdry |
10 mM N-p-hydroxyphenyl-carbamoylglycine,
50 mM KiPO4, pH 7.0, 37°C |
